第 1 季 共 13 集
播出时间：2008年3月9日
第 1 集 Survival of the Fittest
播出时间：2008年3月9日
Summer is over for Peter Parker and school's ready to start -- both literally (it's his junior year of high school), and in the real world of crime-fighting -- as Spider-Man faces his first real challenge (The Enforcers) and his first real super-villain (Vulture).
第 2 集 Interactions
播出时间：2008年3月9日
Peter Parker attempts to tutor the popular Liz Allan while Spider-Man tries to stop Electro, who will stop at nothing to find a cure for the accident that made him who he is.
第 3 集 Natural Selection
播出时间：2008年3月16日
Peter Parker and Spider-Man both must learn to own their choices when decisions made by Dr. Curt Connors transform him into the Lizard.
第 4 集 Market Forces
播出时间：2008年3月23日
While Peter takes on the responsibility of helping Aunt May pay the bills, Montana (one of the Big Man's Enforcers) becomes the stunning Shocker to fulfill his responsibility: eliminating Spider-Man.
第 5 集 Competition
播出时间：2008年3月30日
As Peter Parker and Harry Osborn try out for the football team, Spider-Man must face convict Flint Marko, newly transformed into the Sandman in order to destroy Spider-Man.
第 6 集 The Invisible Hand
播出时间：2008年4月13日
Peter Parker struggles to get a date for the Fall Formal, and to figure out a way to pay for it. Meanwhile, the unstoppable Rhino – the latest of the Big Man's creations – has sworn vengeance on Spider-Man. Spidey takes the battle directly to the source of his troubles, the previously invisible hand, who has been secretly conducting his villain-of-the-week attacks. But the Big Man offers a surprising solution to Spidey's problems, a tempting offer he doesn't think our hero can afford to refuse. Will Spidey do the right thing?
第 7 集 Catalysts
播出时间：2008年4月27日
On the night of the Midtown High Fall Formal, Peter's date, Mary Jane Watson, threatens to invert Midtown's social structure. Elsewhere, the mysterious, murderous Green Goblin tries to force L. Thompson Lincoln (a.k.a. Tombstone) to step down as NYC's Big Man of crime. Will Spider-Man be able to take this new threat seriously enough – or will he be so cocky that he loses his big head?
第 8 集 Reaction
播出时间：2008年5月4日
While Colonel John Jameson struggles to land his damaged space shuttle, Spider-Man struggles to defeat the Green Goblin, who also threatens Hammerhead and Tombstone. But Peter Parker's biggest challenge will be facing the truth when he finally learns the Goblin's true identity, all on Halloween.
第 9 集 The Uncertainty Principle
播出时间：2008年5月11日
While Colonel John Jameson struggles to land his damaged space shuttle, Spider-Man struggles to defeat the Green Goblin, who also threatens Hammerhead and Tombstone. But Peter Parker's biggest challenge will be facing the truth when he finally learns the Goblin's true identity, all on Halloween.
第 10 集 Persona
播出时间：2008年5月18日
Spider-Man gains a black costume, courtesy of the alien life form, during an encounter with the Black Cat. Meanwhile, a master of disguise, the Chameleon, starts framing Spidey for various robberies, using inventions and effects provided by Quentin Beck and Phineas Mason. Spider-Man is left with no option but to team up with Black Cat to stop his new enemy's crime spree.
第 11 集 Group Therapy
播出时间：2008年6月1日
With Electro's help, Doctor Octopus breaks himself, Vulture, Sandman, Rhino, and Shocker out of Ryker's Island prison to quench their mutual thirst for revenge. Even with his new black alien suit, Spider-Man finds his old enemies, now called the Sinister Six, are more than he can handle.
第 12 集 Intervention
播出时间：2008年6月8日
With Aunt May in the hospital and his friendship with Eddie Brock ruined, Peter finally gains enough self-awareness to realize the alien symbiote is starting to affect his personality. The struggle between Peter and the symbiote evolves into a war within Spidey's own mind, where Peter is aided by a familiar face.
第 13 集 Nature vs. Nurture
播出时间：2008年6月15日
Peter is in a celebratory mood as Aunt May is released from the hospital, but Eddie Brock (transformed into the vicious Venom by the alien symbiote) has no intention of letting them have a happy reunion. Can Spider-Man defeat his new arch-nemesis?
