第 2 季 共 13 集
播出时间：2009年6月23日
第 1 集 Blueprints
播出时间：2009年6月23日
It’s early December, and the first snow is falling. Peter Parker has a long “to do” list – starting with talking to Gwen Stacy about their surprise first kiss. But before he gets the chance, Spider-Man has a run-in with the sorcerer Mysterio.
第 2 集 Destructive Testing
播出时间：2009年6月23日
Both Gwen Stacy and Liz Allan like Peter Parker. So whom should Peter pursue? Meanwhile, Kraven the Hunter has come to New York to make Spider-Man his prey.
第 3 集 Reinforcement
播出时间：2009年6月30日
As Christmas approaches, the Sinister Six reconstitutes with Sandman, Rhino, Vulture, Electro, and new members Mysterio and Kraven. All they want for Christmas is Spider-Man — dead. Meanwhile, Peter Parker finds his wandering eye prevents him from being with Gwen Stacy. He’ll have to learn to focus if he wants to get the girl, and if he wants to stay alive.
第 4 集 Shear Strength
播出时间：2009年7月7日
It’s New Year’s Eve, and the Master Planner and his henchmen are planning to take over the world. A highly motivated Spider-Man takes the fight to the Planner’s secret lair, but once he gets there, can he survive?
第 5 集 First Steps
播出时间：2009年7月14日
School’s back in session, and things have changed. Peter Parker’s actually invited to Flash Thompson’s birthday bash. Meanwhile, Spider-Man has to party with Sandman, who’s once again looking to make his Big Score. Harry Osbourne shows up at school again. And is that Venom disappearing into the night?
第 6 集 Growing Pains
播出时间：2009年7月21日
Venom is back and framing Spider-Man. Meanwhile, alien spores infect John Jameson, increasing his size, strength and mass. J. Jonah Jameson convinces his son to become a superhero, to capture Spidey.
第 7 集 Identity Crisis
播出时间：2009年7月28日
Peter Parker’s secret identity as Spider-Man has been outed by Venom, leaving Peter and his loved ones vulnerable to all of Spidey’s enemies. At the end, Spider-Man separated Eddie and Venom. Eddie gets treatment, but Venom's on the loose in the sewer tunnels.
第 8 集 Accomplices
播出时间：2009年10月8日
A gang war looms as Silver Sable, Hammerhead, and Doctor Octopus vie for a prize that could change the balance of power in the New York Underworld. Spidey finds himself in the thick of this fray and must rely on help with surprising allies to stay alive....
第 9 集 Probable Cause
播出时间：2009年10月15日
Some days, Peter Parker just can’t win, especially when he winds up on a Midtown High police car ride, along with Sally Avril. As Spider-Man, his luck isn’t any better, facing off against Shocker, Ricochet, and Ox, the new Enforcers.
第 10 集 Gangland
播出时间：2009年10月22日
On Valentine’s Day, Peter Parker jeopardizes his relationship with girlfriend Liz Allan when Tombstone, Doctor Octopus, and Silvermane call a Valentine’s Day Summit. But when Hammerhead betrays them all in an attempt to become the new Big Man, the summit erupts into a gang war – one only Spider-Man can stop.
第 11 集 Subtext
播出时间：2009年11月5日
Determined to be a better boyfriend to Liz Allan, Peter Parker wishes he could help her brother Mark. But when Green Goblin turns Mark into Molten Man and blackmails him into doing his bidding, neither Liz, Mary Jane Watson, nor Spider-Man may be able to save him.
第 12 集 Opening Night
播出时间：2009年11月19日
Spider-Man volunteers to try to escape the Vault in order to test the security system. But the Green Goblin has other ideas, trapping Spidey inside Ryker’s amid a sea of felons he put away.
第 13 集 Final Curtain
播出时间：2009年11月19日
When Peter Parker breaks up with Liz Allan to finally be with Gwen Stacy, nothing goes as planned. Likewise for Spider-Man, who finally unmasks the Green Goblin.
